Semaglutide

What is Semaglutide?

Semaglutide is a synthetic 31-amino-acid acylated peptide, an analogue of GLP-1(7-37), with CAS registry number 910463-68-2 and molecular formula C187H291N45O59. The literature classifies it as a GLP-1 receptor agonist based on receptor studies. Nocap Research supplies it as a lyophilized reference material for laboratory research only.

Chemical Identity

Semaglutide, also referenced by the development code NN9535, is a synthetic peptide composed of 31 amino acids and designed as an analogue of glucagon-like peptide-1 (7-37). It is catalogued under CAS registry number 910463-68-2 and is described by the molecular formula C187H291N45O59, with a molecular weight of approximately 4113.6 g/mol. In the pharmacological literature the molecule is classified as a GLP-1 receptor agonist. These are molecular characterizations and do not describe an effect in humans or animals. Nocap Research offers this compound solely as a reference material for in vitro laboratory investigation.

Specifications

Structure and Modifications

Semaglutide differs from native GLP-1(7-37) in three places: an Aib residue at position 2 (position 8 in GLP-1 numbering), an arginine at position 28 (position 34), and an acylated lysine at position 20 (position 26) that carries a C18 fatty diacid attached through a gamma-glutamic acid and two oligo(ethylene glycol) linker units. The C-terminus is a free acid.

The medicinal chemistry behind these substitutions is described in the original discovery paper, and cryo-EM structures of the peptide bound to the GLP-1 receptor have since been published.

Physical Form, Handling, and Storage

The material is supplied as a lyophilized (freeze-dried) powder. As a laboratory reagent, lyophilized peptide is generally stored desiccated and protected from light, with long-term storage commonly at -20C or below and short-term working material at 2C to 8C. For laboratory work the powder is solubilized in a solvent chosen for the intended in vitro application. These notes describe general handling of the material as a research reagent. They are not instructions for use in humans or animals.

Purity and Certificate of Analysis

Every Nocap Research batch of Semaglutide is tested by an independent laboratory before it is listed. The current batch results are below, transcribed from the certificate of analysis. The full certificate, and a link to verify it directly with the lab, are one click away.

How Nocap Research tests every batch and how to read a certificate of analysis.

Selected Scientific Literature

The peer-reviewed papers below characterize Semaglutide at the level of chemistry, structure, synthesis or analytical method. These references describe scientific literature and are not evidence of safety or efficacy for use in humans or animals.

1. Lau J et al. Discovery of the Once-Weekly Glucagon-Like Peptide-1 (GLP-1) Analogue Semaglutide. J Med Chem. 2015. Describes the medicinal chemistry that produced the acylated GLP-1 analogue. PMID 26308095 DOI 10.1021/acs.jmedchem.5b00726
2. Zhang X et al. Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes. Cell Rep. 2021. Reports cryo-EM structures of the peptide bound to its receptor. PMID 34260945 DOI 10.1016/j.celrep.2021.109374
3. Knudsen LB et al. The Discovery and Development of Liraglutide and Semaglutide. Front Endocrinol (Lausanne). 2019. Reviews the design rationale and chemistry of two acylated GLP-1 analogues. PMID 31031702 DOI 10.3389/fendo.2019.00155

Search PubMed for more Semaglutide literature

Semaglutide 10mg in the Catalog

Price, current stock and the batch certificate for Semaglutide 10mg are listed on its product page. An account is required to purchase.

View Semaglutide 10mg: price, stock and certificate of analysis

More in the Research Library